The Roman Catholic Diocese of Juigalpa (erected 21 July 1962, as the Territorial Prelature of Juigalpa) is a suffragan of the Archdiocese of Managua. It was elevated on 30 April 1991.
Ordinaries
- Julián Luis Barni Spotti, O.F.M. (1962–1970), appointed Bishop of Matagalpa
- Pablo Antonio Vega Mantilla (1970–1993)
- Bernardo Hombach Lütkermeier (1995–2003), appointed Bishop of Granada
- Sócrates René Sándigo Jiron (2004–2019), appointed Bishop of León en Nicaragua
- Marcial Humberto Guzmán Saballo (2020–)